Description
NucleoCounter® NC-3000™
- A variety of fixed and build-your-own assays
- High-speed cell counting and advanced data analysis
- No warm-up or calibration required
- No liquid waste produced
- 21 CFR Part 11/GMP-ready software
NucleoCounter® NC-3000™
NucleoCounter® NC-3000™
- A variety of fixed and build-your-own assays
- High-speed cell counting and advanced data analysis
- No warm-up or calibration required
- No liquid waste produced
- 21 CFR Part 11/GMP-ready software
Technical Specification
Applications: Fixed assays: Cell count & viability, Cell cycle, Mitochondrial potential, Vitality, Caspase, Annexin V, Transfection efficiency, and DNA fragmentation assays. User-adapted protocols with the Flexicyte™ module
Cell types: Mammalian, yeast, insect, avian
Software: NucleoView™
Consumables: Via1-Cassette™, NC-Slide A2™ and NC-Slide A8™
Analysis time: Variable, depending on consumable and assay type
Volume needed: 60 μl (Via1-Cassette™), 30 μl (NC-Slide A2™), 10 μl (NC-Slide A8™)
Optimal range: 5×104 to 5×106 cells/ml (for counting)
Staining dyes: Hoechst 33342, JC-1, VitaBright-43™, VitaBright-48™, Acridine orange (AO), DAPI, Propidium iodide (PI), User-defined
Excitation: Seven LED light sources with peaks at 365 nm, 405 nm, 475 nm, 530 nm, 630 nm + 2 white LEDs
Emission: Nine interchangeable emission filters: 430/20, 470/55, 475/15, 530/15, 560/35, 580/25, 675/75, 630 LP, 740/60
Optics: Lens with x2 magnification, 1/2″ CCD camera with 1392×1040 pixels
Size: 29 x 29 x 31 cm (W x H x D)
Weight: 14 kg (30.9 lb)
Lead time
2 – 4 weeks
Country of Origin
Denmark
Additional Information
The NucleoCounter® NC-3000™ is an advanced image cytometer that offers up to 4 different fluorescent channels to image and study your cells. The instrument performs high-speed analytical assays of mammalian, yeast, avian and insect cells, including a unique 5-minute cell cycle assay, several apoptosis assays, and the standard cell count and viability assay.
With the FlexiCyte™ license, users can create customized protocols with options for light source, emission filters, and other detailed settings. The NucleoView™ software, which comes with unlimited licenses, is ideal for intuitive data analysis. Users can explore their data in flow cytometry style dot plots and are able to see the corresponding images in real time.
This image cytometer ensures highly reproducible flow-like analysis, requires low sample volume and does not require calibration. When used with the disposable Via1-Cassette™, it creates a closed cell counting system that helps keep users protected when handling samples. The NucleoCounter® NC-3000™ is a safe and reliable option, requiring no pipettes, no exposure to toxic reagents, and minimal clean-up.
Please note that the FlexiCyte™ protocols are also compatible with the NC-Slides A2TM for a higher throughput. The NC-slides A8TM can be used for protocols not requiring the FlexiCyte™ license.
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